Journal: PLoS ONE

Article Title: Accurate and reproducible enumeration of T-, B-, and NK lymphocytes using the BD FACSLyric 10-color system: A multisite clinical evaluation

doi: 10.1371/journal.pone.0211207

Figure Lengend Snippet: T-, B-, and NK cell Deming regression results determined using BD Multitest reagents.

Article Snippet: The BD Multitest 6-color TBNK reagent (CD3 FITC / CD16 PE + CD56 PE / CD45 PerCP-Cy5.5 / CD4 PE-Cy7 / CD19 APC / CD8 APC-Cy7) comprised a single tube.

Techniques:

Journal: PLoS ONE

Article Title: Accurate and reproducible enumeration of T-, B-, and NK lymphocytes using the BD FACSLyric 10-color system: A multisite clinical evaluation

doi: 10.1371/journal.pone.0211207

Figure Lengend Snippet: This is the Fig 2 legend. The T- cells (CD3 + , CD3 + CD4 + and CD3 + CD8 + ) shown as A, B, and C; the NK lymphocytes in D, and the B-lymphocytes are depicted in E stained with BD Multitest 6-Color TBNK reagent. The Deming regression are the upper plots with identity line (grey) and the Weighted Deming regression line (red) of each subset. The x-axis depicts BD FACSCanto II cell counts and the y-axis shows the BD FACSLyric counts. The Bland-Altman graphs are the lower plots of each subset, with the bias line (blue solid line) and the upper and lower limits of agreement (blue dotted lines). For these plots, the x-axis corresponds to the average of the lymphocyte marker, and the y-axis represents the estimated percent difference of the absolute counts between systems.

Article Snippet: The BD Multitest 6-color TBNK reagent (CD3 FITC / CD16 PE + CD56 PE / CD45 PerCP-Cy5.5 / CD4 PE-Cy7 / CD19 APC / CD8 APC-Cy7) comprised a single tube.

Techniques: Staining, Marker

Journal: PLoS ONE

Article Title: Accurate and reproducible enumeration of T-, B-, and NK lymphocytes using the BD FACSLyric 10-color system: A multisite clinical evaluation

doi: 10.1371/journal.pone.0211207

Figure Lengend Snippet: Agreement at the CD4 clinical cutoff of 200 cells/μL.

Article Snippet: The BD Multitest 6-color TBNK reagent (CD3 FITC / CD16 PE + CD56 PE / CD45 PerCP-Cy5.5 / CD4 PE-Cy7 / CD19 APC / CD8 APC-Cy7) comprised a single tube.

Techniques:

Journal: PLoS ONE

Article Title: Accurate and reproducible enumeration of T-, B-, and NK lymphocytes using the BD FACSLyric 10-color system: A multisite clinical evaluation

doi: 10.1371/journal.pone.0211207

Figure Lengend Snippet: Predicted bias interval at the CD4 clinical cutoff.

Article Snippet: The BD Multitest 6-color TBNK reagent (CD3 FITC / CD16 PE + CD56 PE / CD45 PerCP-Cy5.5 / CD4 PE-Cy7 / CD19 APC / CD8 APC-Cy7) comprised a single tube.

Techniques:

Journal: PLoS ONE

Article Title: Accurate and reproducible enumeration of T-, B-, and NK lymphocytes using the BD FACSLyric 10-color system: A multisite clinical evaluation

doi: 10.1371/journal.pone.0211207

Figure Lengend Snippet: Inter-laboratory total precision lymphocyte subset results with BD Multitest 6-color TBNK reagent.

Article Snippet: The BD Multitest 6-color TBNK reagent (CD3 FITC / CD16 PE + CD56 PE / CD45 PerCP-Cy5.5 / CD4 PE-Cy7 / CD19 APC / CD8 APC-Cy7) comprised a single tube.

Techniques:

Journal: PLoS ONE

Article Title: Accurate and reproducible enumeration of T-, B-, and NK lymphocytes using the BD FACSLyric 10-color system: A multisite clinical evaluation

doi: 10.1371/journal.pone.0211207

Figure Lengend Snippet: The variability per site is illustrated with the BD Multitest 6-color TBNK reagent total precision. The total precision results using CD Chex Plus control materials for the CD4 low are shown on the upper plot, and the total precision for CD4 normal on the bottom plot. The x-axis shows the participating laboratoriers per number and color. The y-axis displays the percentage of coefficient of variance (%CV) and the upper CV limit.

Article Snippet: The BD Multitest 6-color TBNK reagent (CD3 FITC / CD16 PE + CD56 PE / CD45 PerCP-Cy5.5 / CD4 PE-Cy7 / CD19 APC / CD8 APC-Cy7) comprised a single tube.

Techniques:

Journal: PLoS ONE

Article Title: Accurate and reproducible enumeration of T-, B-, and NK lymphocytes using the BD FACSLyric 10-color system: A multisite clinical evaluation

doi: 10.1371/journal.pone.0211207

Figure Lengend Snippet: Specimen and stained sample storage biases at different time points using BD Multitest reagents.

Article Snippet: The BD Multitest 6-color TBNK reagent (CD3 FITC / CD16 PE + CD56 PE / CD45 PerCP-Cy5.5 / CD4 PE-Cy7 / CD19 APC / CD8 APC-Cy7) comprised a single tube.

Techniques: Staining

Journal: PLoS ONE

Article Title: Accurate and reproducible enumeration of T-, B-, and NK lymphocytes using the BD FACSLyric 10-color system: A multisite clinical evaluation

doi: 10.1371/journal.pone.0211207

Figure Lengend Snippet: Lymphocyte subset reference intervals for the BD Multitest reagents.

Article Snippet: The BD Multitest 6-color TBNK reagent (CD3 FITC / CD16 PE + CD56 PE / CD45 PerCP-Cy5.5 / CD4 PE-Cy7 / CD19 APC / CD8 APC-Cy7) comprised a single tube.

Techniques:

Journal: Cells

Article Title: Chronic Pain Induced by Social Defeat Stress in Juvenile Mice Depends on TLR4

doi: 10.3390/cells14050350

Figure Lengend Snippet: SDS increases TLR4 dimers of spinal cord microglia and DRG macrophages. ( A ) TLR4 dimers (%) in SDS and no-SDS groups of microglia (CD11b + population) and ( B ) macrophages (CD11b + /F4-80 + population) on day 22 of the protocol. ( C ) Gating strategy for lumbar spinal cord cells, first live cells were gated (APC-Cy7 live/dead Ghost dye); second, microglia population (PercpCy 5.5-conjugated CD11b+), lastly—TLR4 monomers (PC7-conjugated TLR4/MD2) or TLR4 total (APC-conjugated total TLR4). ( D ) Gating strategy for dorsal root ganglia cells, first live cells were gated (APC-Cy7 live/dead Ghost dye); second, microglia population (PercpCy 5.5-conjugated CD11b+), third macrophages population (488-conjugated F4-80) and lastly—TLR4 monomers (PC7-conjugated TLR4/MD2) or TLR4 total (APC-conjugated total TLR4). Data represent mean ± SEM, t -test, SDS vs. no-SDS, * p < 0.05, ** p < 0.01, n = 6 (SDS), and n = 6 (no-SDS).

Article Snippet: Single-cell suspensions were incubated for 45 min on ice with the following antibodies: APC-Cy7 live/dead Ghost dye (#18452, Cell Signaling, Danvers, MA, USA) or 540 live/dead Ghost dye (#72086, Cell Signaling, Danvers, MA, USA); PC7-conjugated TLR4/MD2 (#MTS510; ThermoFisher, Waltham, MA, USA); APC-conjugated total TLR4 (#SA15-21; Biolegend, San Diego, CA, USA); 488-conjugated F4-80 (#123110; Biolegend, San Diego, CA, USA); and PercpCy 5.5-conjugated CD11b (#101228; Biolegend, San Diego, CA, USA).

Techniques: